Identification Of Bacteria: Morphology And Staining Reactions
Since we are studying bacteria. It become important to identify its type, species or strain to continue our documentation or
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staining
Leifson staining method of flagella
Requirements Bacterial culture (Pseudomonas fluorescens) Pipette Formaline Centrifuge Inoculation needle Bunsen burner Microscope Glass slide Coveralls Prepare the following reagents:
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Gray staining method for flagella
Many bacteria are mobile due to the presence of one or more very fine thread-like, filaments structures called flag Ella’s,
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Demonstration of Staining of Cysts
Some bacteria such as Azotobacter sp. contains cysts which are visible with following reagent. Requirements Glacial acetic acid 8.5 ml
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Dorner-staining method for bacterial endospores
Species at Bacillus Clostridium, Desulfotomaculum, Sporosarcina, etc. have the ability to form thick walled oval body. These cells have large
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Negative staining of endospores
Negative staining is one of the most popular methods to stain endospores in bacteria. The coloring power of acidic dye
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Popping test for endospore staining
Endospores are dormant structures formed in various bacteria under unfavourable conditions. Their size is irregular and highly refractile and heat-resistant.
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Ziehl Neelsen staining method
Ziehl Neelsen staining method is commonly used for staining of acid fast bacteria. In Mycobacterium and some species of Nocardia,
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Acid fast staining of Mycobacterium
Certain bacteria (Mycobacterium spp.) and actinomycetes have waxy components of the cell wall, hence their cell wall has limited permeability.
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Gram’s Staining for differentiation of bacteria
Gram’s staining is one of the most important differential techniques used in bacteriology. There are two groups: gram positive and
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