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staining

VP Test
Microbiology 

Voges – Proskauer Test

November 21, 2022November 20, 2022 Gaurav Singh 0 Comments bacteria, bacteria identification, Isolation of microorganism, staining

Voges – Proskauer or VP test is used as one of the key test in identification of bacterial culture. This

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bacillus
Bacteriology 

Bacillus

September 5, 2022September 5, 2022 Gaurav Singh 0 Comments aerobic bacteria, anaerobic bacteria, bacteria, Grams staining, staining

Bacillus is a genus of rod-shaped aerobic bacteria forming heat resistant sporesa. If applied by Gram stain they can easily

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identification of bacteria
Microbiology 

Identification Of Bacteria: Morphology And Staining Reactions

April 4, 2022April 4, 2022 Gaurav Singh 0 Comments bacteria, bacteria identification, morphology of bacteria, staining, staining standerization

Since we are studying bacteria. It become important to identify its type, species or strain to continue our documentation or

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Flagella staining
Bacteriology Microbiology 

Leifson staining method of flagella

November 8, 2021November 8, 2021 Gaurav Singh 0 Comments bacteria, bacteria identification, Flagella staining, microbiology, staining

Requirements Bacterial culture (Pseudomonas fluorescens) Pipette Formaline Centrifuge Inoculation needle Bunsen burner Microscope Glass slide Coveralls Prepare the following reagents:

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Flagella staining
Bacteriology Microbiology 

Gray staining method for flagella

November 7, 2021November 7, 2021 Gaurav Singh 0 Comments bacteria, bacteria identification, differential staining, Flagella staining, microbiology, staining

Many bacteria are mobile due to the presence of one or more very fine thread-like, filaments structures called flag Ella’s,

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Bacterial cysts
Bacteriology Microbiology 

Demonstration of Staining of Cysts

October 31, 2021October 31, 2021 Gaurav Singh 0 Comments bacteria, bacteria identification, Bacterial cysts, cysts, microbiology, mycobacterium, staining

Some bacteria such as Azotobacter sp. contains cysts which are visible with following reagent. Requirements Glacial acetic acid 8.5 ml 

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Negative staining
Bacteriology Microbiology 

Dorner-staining method for bacterial endospores

October 24, 2021October 24, 2021 Gaurav Singh 0 Comments bacteria, bacteria identification, differentiation staining, Endospores staining, Grams staining, microbiology, staining

Species at Bacillus Clostridium, Desulfotomaculum, Sporosarcina, etc. have the ability to form thick walled oval body. These cells have large

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Negative staining
Bacteriology Microbiology 

Negative staining of endospores

October 23, 2021October 23, 2021 Gaurav Singh 0 Comments bacteria, differential staining, Endospores staining, microbiology, Negative staining, staining

Negative staining is one of the most popular methods to stain endospores in bacteria. The coloring power of acidic dye

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Endospores staining
Bacteriology Microbiology 

Popping test for endospore staining

October 22, 2021October 22, 2021 Gaurav Singh 0 Comments bacteria, bacteria identification, differential staining, differentiation staining, Endospores staining, microbiology, mycobacterium staining, staining

Endospores are dormant structures formed in various bacteria under unfavourable conditions. Their size is irregular and highly refractile and heat-resistant.

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ZN Staining
Bacteriology Microbiology 

Ziehl Neelsen staining method

October 16, 2021October 16, 2021 Gaurav Singh 0 Comments bacteria, bacteria identification, differentiation staining, microbiology, mycobacterium, mycobacterium staining, staining, Ziehl neelsen staining, ZN staining

Ziehl Neelsen staining method is commonly used for staining of acid fast bacteria. In Mycobacterium and some species of Nocardia,

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