RDT

Recombinant DNA Technology

Recombinant DNA technology, a pivotal advancement in the field of molecular biology, involves the manipulation and combination of genetic material from different sources to create novel DNA sequences. This revolutionary technique has empowered scientists to isolate, modify, and insert specific genes into host organisms, leading to the production of proteins, enzymes, and even entire organisms with desired traits. The process entails cutting DNA using restriction enzymes, which act as molecular scissors, and then splicing together the fragments with the help of DNA ligases. This technology has applications ranging from the development of genetically modified organisms (GMOs) for improved agriculture and pharmaceutical production to the creation of recombinant proteins used in medical treatments and diagnostics. It has not only deepened our understanding of genetics but also sparked ethical debates and regulatory considerations regarding the potential risks and benefits associated with its implementation.