Demonstration of Staining of Cysts
Some bacteria such as Azotobacter sp. contains cysts which are visible with following reagent. Requirements Glacial acetic acid 8.5 ml
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mycobacterium
Ziehl Neelsen staining method
Ziehl Neelsen staining method is commonly used for staining of acid fast bacteria. In Mycobacterium and some species of Nocardia,
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Acid fast staining of Mycobacterium
Certain bacteria (Mycobacterium spp.) and actinomycetes have waxy components of the cell wall, hence their cell wall has limited permeability.
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