Immunoelectroblot Techniques 

Immunoelectroblot or electroimmunoblot techniques combine the sensitivity of enzyme immunoassay with much greater specificity.

The technique is a combination of three separate procedures:

  • Separation of ligand-antigen components by polyacrylamide gel electrophoresis,
  • Blotting of the electrophoresed ligand fraction on nitrocellulose membrane strips
  • Enzyme immunoassay or radioimmunoassay to
    • Detect antibody in test sera against the various ligand fraction bands; or
    • Probe with known anti sera against specific antigen bands.

The western Blot test, considered the definitive test for the serodiagnosis of HIV infection, is an example of the immunoelectroblot tech.

Gaurav Singh

Editor in Chief Medical Microbiology & Recombinant DNA Technology (RDT) Labs - RDT Labs Magazine

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