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Month: October 2021

Bacterial cysts
Bacteriology Microbiology 

Demonstration of Staining of Cysts

October 31, 2021October 31, 2021 Gaurav Singh 0 Comments bacteria, bacteria identification, Bacterial cysts, cysts, microbiology, mycobacterium, staining

Some bacteria such as Azotobacter sp. contains cysts which are visible with following reagent. Requirements Glacial acetic acid 8.5 ml 

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Negative staining
Bacteriology Microbiology 

Dorner-staining method for bacterial endospores

October 24, 2021October 24, 2021 Gaurav Singh 0 Comments bacteria, bacteria identification, differentiation staining, Endospores staining, Grams staining, microbiology, staining

Species at Bacillus Clostridium, Desulfotomaculum, Sporosarcina, etc. have the ability to form thick walled oval body. These cells have large

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Negative staining
Bacteriology Microbiology 

Negative staining of endospores

October 23, 2021October 23, 2021 Gaurav Singh 0 Comments bacteria, differential staining, Endospores staining, microbiology, Negative staining, staining

Negative staining is one of the most popular methods to stain endospores in bacteria. The coloring power of acidic dye

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Endospores staining
Bacteriology Microbiology 

Popping test for endospore staining

October 22, 2021October 22, 2021 Gaurav Singh 0 Comments bacteria, bacteria identification, differential staining, differentiation staining, Endospores staining, microbiology, mycobacterium staining, staining

Endospores are dormant structures formed in various bacteria under unfavourable conditions. Their size is irregular and highly refractile and heat-resistant.

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ZN Staining
Bacteriology Microbiology 

Ziehl Neelsen staining method

October 16, 2021October 16, 2021 Gaurav Singh 0 Comments bacteria, bacteria identification, differentiation staining, microbiology, mycobacterium, mycobacterium staining, staining, Ziehl neelsen staining, ZN staining

Ziehl Neelsen staining method is commonly used for staining of acid fast bacteria. In Mycobacterium and some species of Nocardia,

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Acid Fast Staining
Bacteriology Microbiology 

Acid fast staining of Mycobacterium

October 16, 2021October 16, 2021 Gaurav Singh 0 Comments Acid fast staining, bacteria, differential staining, microbiology, mycobacterium, mycobacterium staining, staining

Certain bacteria (Mycobacterium spp.) and actinomycetes have waxy components of the cell wall, hence their cell wall has limited permeability.

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Bacteriology Microbiology 

Gram’s Staining for differentiation of bacteria

October 14, 2021October 14, 2021 Gaurav Singh 0 Comments bacteria identification, differentiation staining, Grams staining, microbiology, staining

Gram’s staining is one of the most important differential techniques used in bacteriology. There are two groups: gram positive and

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